MI 1.1.3 PCR Pre-Lab Qs F25

Which Nobel prize did Dr. Mullis win for his development of PCR as a lab technique?

• Biology

• Medicine

• Chemistry

• Peace

Which type of DNA is best to use in a PCR protocol?

• recently isolated

• chemically preserved

• cryogenically frozen

Where do primers come from?

• they are synthesized in a lab

• they are isolated from cheek cells

• they exist in nature

• isolated from bacteria only

Primers are designed to be complementary to the ​ (a)   of the target DNA sequence.

• ends
• middle
• inside

DNA (a)   enzyme adds new nucleotides to a growing DNA strand.

DNA polymerase always adds free nucleotides in the​ (a)   to (b)   direction.

• 3-prime
• 5-prime

If a template DNA sequence reads AATGCGC, a primer that could bind to it might read

• TACG

• AATGCGC

• TTAGCGC

• CGCGTAA

Which of the following is NOT an ingredient in the PCR reaction tube?

• an excess of primers

• free deoxynucleotides

• DNA polymerase

• template DNA

• Calcium buffer solution

Where does Taq polymerase come from?

• a hot springs bacterium

• human cells

• the nucleus

• frogs

Thermocycling means

• heating and cooling several times on repeat

• heating to a high temp for the duration of the reaction

• choosing either hot or cold for the reaction

• putting the bacteria in and out of the hot springs

Tags

NGSS.HS-PS1-5