Gene Cloning

Restriction enzymes are analogous to what common school supply?

• glue

• scissors

• paper

• highlighter

Restriction Enzymes can cut at any sequence of DNA

• True

• False

The site where restriction enzymes cut are a called:

• cut sites

• restriction sites

• recognition sites

• cleave sites

Where are restriction enzymes naturally found?

• in humans

• in bacteria

• in algae

• they are not natural - all restriction enzymes are made in the lab

What is a plasmid?

• a section of the human genome

• a material that cuts DNA

• viral DNA

• an accessory piece of DNA found in bacteria

If a linear piece of DNA contains 3 recognition sites for a restriction enzyme. How many pieces will be produced when the restriction enzyme cuts the DNA?

• 2

• 3

• 4

• 5

• 6

Sticky ends are produced when the restriction enzyme cuts in a:

• staggered manner

• straight through the DNA

A plasmid is larger than a bacterial genome

• True

• False

Plasmids often have genes that give the bacterial a survival advantage

• True

• False

Tags

NGSS.HS-LS4-2

The multiple cloning site in the plasmid only allows one restriction enzyme to be able to cut open the plasmid

• True

• False