Genetic Technology Middle School

When an organism is genetically engineered, what occurs?

• the genome is identical to their parents'

• they are made using half the genomes of two different species

• the organism contains genes that were transferred from another organism

• they are produced by breeding only the individuals with desired traits.

A biologist needs to know which of three male zebras fathered the litter. Which process can solve this problem.

• organism cloning

• genome sequencing

• gene therapy

• DNA fingerprinting

A child is born with a faulty gene that causes a serious disease. Which process can solve this problem.

• organism cloning

• genome sequencing

• gene therapy

• DNA fingerprinting

Restriction enzymes are analogous to what common school supply?

• glue

• scissors

• paper

• highlighter

Restriction Enzymes can cut at any sequence of DNA

• True

• False

What is a plasmid?

• a section of the human genome

• a material that cuts DNA

• viral DNA

• an accessory piece of DNA found in bacteria

Sticky ends are produced when the restriction enzyme cuts in a:

• staggered manner

• straight through the DNA

A plasmid is larger than a bacterial genome

• True

• False

How many times do you want a restriction enzyme to cut a human genome in order to cut out the gene of interest to insert into a plasmid?

• 1 - through the gene

• 2 - on either side of the gene

• 3 - through the gene and on either side of the gene

• 4 - twice on each side of the gene

What enzyme is used to ensure that the recombinant DNA created is bonded together along the sugar-phosphate backbone?

• DNA polymerase

• RNA polymerase

• ligase

• helicase