Restriction Enzymes

What kind of cuts were made in the scenario above?

• Blunt

• Sticky

• No cuts were made

• Palindrome

Restriction enzymes aid in creating genetically modified organisms to cut out the desired gene and host DNA.

• true

• false

Which restriction enzyme listed below was isolated from E.coli bacteria?

• AluI

• HindIII

• HaeII

• EcoRI

Why do bacteria have restriction enzymes?

• To prevent being infected by viruses

• To help DNA get into the cell

• To be able to grow in an incubator

• They don't - viruses have restriction enzymes

Restriction enzymes produce random cuts along the DNA.

• True

• False

If the target sequence or restriction site is ^5'GCTTCG^3', what is the complementary sequence read in the 3'-5' direction?

• CGAAGC

• GCTTCG

• CGUUCG

• GCUUCG

Which is the primary purpose of using restriction enzymes in gel electrophoresis?

• It allows the strands of DNA to be cut into various lengths for testing

• It restricts the number of base pairs that can be tested in a sample

• It makes the testing simpler by moving the strands into the gel faster

• It charges the DNA strands

Which RE's from above make blunt cuts?

• Alul

• HaeIII

• BamHI

• HindIII

• EcorRI

Which RE's from above make sticky cuts?

• Alul

• HaeIII

• BamHI

• HindIII

• EcorRI

What is the primary function of restriction enzymes in molecular biology?

• To cut DNA at specific sequences

• To replicate DNA

• To synthesize proteins

• To transcribe RNA