Chromatography

_________________ utilizes a stationary and a mobile phase to separate mixtures based on their affinity for each phase.

• filtration

• chromatography

• distillation

• osmosis

The diagram below shows a chromatogram obtained when a sample X was analysed together with four other known dyes P, Q, R and S. Dye Q was known to cause cancer. Which of the following dye(s) is/are safe for use?

• P only

• S only

• P and S

• P and X

The mobile phase in paper chromatography is typically a

• plasma

• solid

• gas

• liquid

In the image, which dye is the most soluble?

• Red

• Blue

• Purple

Tags

NGSS.MS-PS1-2

In the image, which dye is the least soluble?

• Red

• Blue

• Purple

Tags

NGSS.HS-PS1-3

The substance in the mixture that has the greatest affinity for the mobile phase will travel

• fastest

• slowest

The retention factor is used

• to zero the chromatography scale

• to identify unknown compounds in a sample

• to retain solvent

• as a starting tool for chromatography

Which type of chromatography is most commonly used for separating proteins?

• Gas chromatography

• Paper chromatography

• Ion-exchange chromatography

• Thin-layer chromatography

In chromatography, what is the role of the stationary phase?

• To move the sample through the system

• To separate components based on their affinity

• To provide energy for the separation process

• To act as a detector for the separated components

What is the main principle behind chromatography?

• Separation based on boiling points

• Separation based on solubility

• Separation based on molecular size

• Separation based on differential affinities