PCR and Gel Electrophoresis

What is the purpose of PCR?

• To produce billions of copies of a specific GENE

• To cut DNA

• To remove DNA from the nucleus

Why do the fragments of DNA in gel electrophoresis travel away from the negative electrode?

• DNA is negatively charged so attracted to the positive end of the unit

• DNA is positively charged to attracted to the negative end of the unit

• the agarose gel in negatively charged

• the agarose gel is positively charged

In gel electrophoresis, the largest DNA fragment will appear

• closest to the starting wells

• farthest from the starting wells

• three quarters away from the starting wells

• it depends on how many fragments there are

Tags

NGSS.HS-PS2-5

Which DNA fragment, A, B, C, D, E, F is the largest?

Which two DNA fragments are the same size?

Which DNA fragmen is about the same size as the lengths of the fragment A and fragment F added together?

• B

• C

• D

• E

What is the purpose of gel electrophoresis in molecular biology?

• To increase the size of DNA fragments

• To separate DNA fragments based on their size

• To create DNA fragments

• To visualize proteins only

The speed at which DNA moves through a gel is diretly related to its size.

• True

• False

Label the the bands correctly.

d

e

f

c

b

a

B2

G

M

D

J

B1

In gel electrophoresis, DNA fragments are separated based on their:

• Shape

• Size

• Charge

• Both B and C

Answer explanation

In gel electrophoresis, DNA fragments are separated based on their size and charge, not their shape. Therefore, the correct answer is Both B and C.