Chapter 12 KUV

Recombinant DNA technology is used:

• For production of herbicide-resistant plant.

• To make a particular protein.

• To clone a specific piece of DNA

• All of the above.

Which of the following is correct about host cell?

• Plasmid is an example of host cell.

• Unable to receive DNA molecule through transformation.

• An organism that prevents cloning vectors from multiplying.

• An organism that is used to receive recombinant DNA for cloning purposes.

What is the purpose of amplification?

• To screen for recombinant bacteria.

• To join the rDNA with the host cell.

• To produce multiple copies of the desired gene.

• To prevent the bacteria from reproducing.

Which of these pairs is incorrectly matched?

• DNA ligase – mapping human chromosomes

• Target DNA – gene of interest

• Taq polymerase – PCR

• Plasmid as a vector – isolated from bacteria

Which of these might be a biotechnology product?

• Vaccine.

• Modified enzyme.

• Protein hormone.

• All of the above.

Plants are more readily manipulated by genetic engineering than are animals because:

• Plant genes do not contain introns.

• A somatic plant cell can often give rise to a complete plant.

• Genes can be inserted into plant cells by microinjection.

• Plant cells have larger nuclei.

The following are steps involved in gene cloning procedures. Rearrange them into the correct sequence:

I. Transformation and amplification.

II. Screen for bacteria that contain recombinant

DNA.

III. Insert DNA fragment into the cut bacterial

plasmid.

IV. Cleave the plasmid and human DNA with the same restriction enzyme.

V. Isolate the bacterial plasmid and human DNA.

• A. I, II, III, IV, V

• B. V, IV, III, I, II

• C. V, IV, III, II, I

• D. IV, V, III, I, II

In genetic engineering, DNA ligase is used as:

• a probe

• a sealing enzyme

• a restriction enzyme

• a mutagen

Which one of the following statements about Restriction Endonuclease is true

• all restriction endonucleases cut DNA at specific sites

• all restriction endonucleases cut DNA at random sites

• all restriction endonucleases join DNA segments at specific sites

• all restriction endonucleases join DNA at random sites

What is the purpose of PCR?

• To produce millions of copies of DNA.
• To produce millions of copies of a specific region of DNA
• To add nucleotides to a DNA sequence
• To watch polymerase work.