Pglo

What is the pGLO plasmid commonly used for in genetic engineering?

• To produce insulin in bacteria

• To create genetically modified plants

• To study gene expression and regulation

• To develop vaccines

Tags

NGSS.HS-LS3-1

NGSS.HS-LS3-2

Which gene in the pGLO plasmid is responsible for antibiotic resistance?

• GFP gene

• araC gene

• bla gene

• ori gene

Tags

NGSS.HS-LS1-1

What is the role of the GFP gene in the pGLO plasmid?

• It provides antibiotic resistance

• It codes for a fluorescent protein

• It regulates the expression of other genes

• It initiates DNA replication

In the pGLO experiment, what is the purpose of adding arabinose to the growth medium?

• To provide nutrients for bacterial growth

• To induce the expression of the GFP gene

• To inhibit bacterial growth

• To enhance antibiotic resistance

What is the function of the araC gene in the pGLO plasmid?

• It acts as a promoter for the GFP gene

• It provides resistance to ampicillin

• It regulates the expression of the GFP gene

• It initiates plasmid replication

Which of the following is a real-world application of the pGLO plasmid?

• Bioremediation of oil spills

• Development of drought-resistant crops

• Production of fluorescent proteins for research

• Synthesis of biofuels

What type of organism is typically transformed with the pGLO plasmid in laboratory experiments?

• Yeast

• Bacteria

• Plants

• Fungi

How is the success of a pGLO transformation experiment typically confirmed?

• By measuring bacterial growth rate

• By observing fluorescence under UV light

• By testing for antibiotic resistance

• By sequencing the plasmid DNA

What is the role of the ori site in the pGLO plasmid?

• It is the origin of replication

• It codes for a fluorescent protein

• It provides antibiotic resistance

• It regulates gene expression

Which of the following conditions is necessary for the expression of the GFP gene in pGLO-transformed bacteria?

• Presence of glucose

• Absence of arabinose

• Presence of arabinose

• Absence of ampicillin