Gel

Which of the following steps of PAGE electrophoresis would come FIRST?

• Pipette protein samples into wells

• Remove cassette from gel

• Immerse gel into stain

• Place casette in gel box at the correct orientation

SDS- PAGE Gel Electrophoresis is used to determine the molecular weight of ____.

Which of the following is a way that a PAGE gel is different than an agarose gel?

• PAGE gels are made of polyacrylamide

• PAGE gels often have a gradient of gel concentrations

• PAGE gels require the use of TBE buffer

• PAGE requires the use of SDS buffer

When placing a PAGE gel cassette in a gel box, the tall side of the cassette should be oriented towards...

• The side of the box with the gel cassette clip

• The middle of the gel box

In gel electrophoresis, the largest DNA fragment will appear

• closest to the starting wells

• farthest from the starting wells

• three quarters away from the starting wells

• it depends on how many fragments there are

What is the purpose of gel electrophoresis in molecular biology?

• To increase the size of DNA fragments

• To separate DNA fragments based on their size

• To create DNA fragments

• To visualize proteins only

Imagine that you have a sample of DNA that contains a single, specific DNA sequence. Before you run your gel, you split your sample into two tubes. You run the DNA from the first tube and use an enzyme called a restriction enzyme that cuts the DNA into two pieces at a specific point in the sequence. You then run this sample that was cut on the gel. Which if the next three lanes could be the same sample from lane two after it was cut into two pieces?

• 3

• 4

• 5

Label the the bands correctly.

d

e

f

c

b

a

G

B1

J

M

D

B2

Where can you find the positive electrode in a vertical gel electrophoresis box?

• At the top

• At the bottom

• On the side

Which of the following is NOT true of the gels used in PAGE electrophoresis?

• They have a concentration of 0.8%

• They allow the smallest proteins to migrate the farthest

• They must be purchased premade

• They are very thin and are therefore stored in a plastic cassette