AP Bio Biotechnology

Where does the heat resistant polymerase come from?

• Fire-resistant plants

• Monkeys

• Nucleus

• Thermophiles

Plasmid makes bacteria resistant to ampicillin and able to synthesize the green glow protein. Which plate will have highest percent of growth?

• Plate with nutrients and no ampicillin present

• Plate with ampicillin and nutrient present

Plasmid makes bacteria resistant to ampicillin and able to synthesize the green glow protein. Which plate will have higher amount of glowing bacteria?

• Plate with nutrients and no ampicillin present

• Plate with ampicillin and nutrient present

Which enzyme was used to produce the molecule in the Figure?

• ligase

• a restriction enzyme

What is the enzymatic function of restriction enzymes?

• to cleave nucleic acids at specific sites

• to add new nucleotides to the growing strand of DNA

The segment of DNA shown in the figure has restriction sites I and II, which create restriction fragments A, B, and C. Which of the gels produced by electrophoresis shown below best represents the separation and identity of these fragments?

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Where does RNA polymerase bind to DNA?

• at an activator site

• on the coding gene sequence

• at a promoter region

• downstream of the coding sequence

• Where the RNA primer is placed

What kind of chemical bond is found between paired bases of the DNA double helix?

• hydrogen

• ionic

• covalent

In an analysis of the nucleotide composition of DNA, which of the following will be found?

• A = G and C = T

• A + C = G + T

What is meant by the description ʺantiparallelʺ regarding the strands that make up DNA?

• The 5ʹ to 3ʹ direction of one strand runs counter to the 5ʹ to 3ʹ direction of the other strand.

• One strand is positively charged and the other is negatively charged.