Microscope Use and Safety

Put the prepared slide on the center of the _______. The specimen should be placed over the hole where the light comes through.

• clips

• stage

• diaphragm

• base

Always start with the ____________ lense.

• medium-power (10X) objective

• low-power (4X) objective

• high-power (40X) objective

• objective

Use the ______________ to bring the low-power objective lens close to the slide. Watch from the side and carefully check to make sure the lense does not touch the side.

• course focus knob

• diaphragm

• fine focus knob

• stage

Adjust the amount of light coming to the specimen, using the ________ located under the stage if you are having trouble viewing the specimen.

• clips

• diaphragm

• fine focus knob

• low-power (4X) objective

When you are ready to increase the microscope's power follow this rule. Once the specimen is in focus, DO NOT turn either of the focus knobs yet. Rotate the ________ into place and now carefully adjust the focus using the ____________.

• low-power (4X) objective and course focus knob

• medium-power (10X) objective and course focus knob

• high-power (40X) objective and fine focus knob

• medium-power (10X) objective and fine focus knob

When you are done, always_____________________.

• take the slide off the stage and clean it as directed

• take the slide off the stage and put it in the slide box as is.

• leave the slide for the next class

• leave the slide and cover the microscope

When you are done, always ___________

• turn on the light

• leave it for the next class

• turn off the light

• leave the light on and cover the microscope

When you are done, always __________________.

• rotate the objectives to the high-power (40X) objective and lower the stage

• rotate the objectives to the medium-power (10X) objective and lower the stage

• rotate the objectives to the low-power (4X) objective and raise the stage

• rotate the objectives to the low-power (4X) objective and lower the stage

When you are done, always_______________.

• leave the microscope uncovered

• cover the microscope with the dustcover

• leave the microscope as is for the next class

• cover the microscope with dust

What is the primary function of the diaphragm on a microscope?

• To hold the slide in place

• To adjust the amount of light reaching the specimen

• To magnify the specimen

• To focus the image