Micropipetting

To pick up DNA with a micropipette, you need to ...

• push the round button to the second stop.

• push the green eject tip button.

• push the round button to the first stop.

• All of the others are correct.

What is the primary purpose of using a micropipette in biotechnology?

• To measure large volumes of liquid

• To pipet very small volumes of liquid with high precision

• To weigh substances accurately

• To create solid solutions

Always change out the micropipette tip when changing reagents.

• true

• false

What is the correct way to set the volume on a micropipette?

• Turn the volume adjustment knob to the desired setting

• Press the plunger to the first stop

• Press the plunger to the second stop

• Eject the tip and reset

Which part of the micropipette is used to draw liquid into the tip?

• The plunger

• The ejector button

• The volume adjustment knob

• The tip holder

What is the typical volume range for a P200 micropipette?

• 0.5-10 µL

• 20-200 µL

• 100-1000 µL

• 200-2000 µL

Why is it important to pre-wet the micropipette tip before use?

• To calibrate the micropipette

• To ensure accuracy and precision

• To sterilize the tip

• To adjust the volume

What should you do if you notice air bubbles in the micropipette tip?

• Ignore them

• Eject the liquid and try again

• Increase the volume setting

• Decrease the volume setting

How should a micropipette be stored when not in use?

• Horizontally on the bench

• Vertically with the tip pointing up

• Vertically with the tip pointing down

• In a drawer

What is the function of the micropipette tip ejector?

• To adjust the volume

• To remove the used tip

• To draw liquid into the tip

• To calibrate the micropipette