DNA Analysis

Why do the fragments of DNA in gel electrophoresis travel away from the negative electrode?

• DNA is negatively charged so attracted to the positive end of the unit

• DNA is positively charged to attracted to the negative end of the unit

• the agarose gel in negatively charged

• the agarose gel is positively charged

In gel electrophoresis, the largest DNA fragment will appear

• closest to the starting wells

• farthest from the starting wells

• three quarters away from the starting wells

• it depends on how many fragments there are

Which is the primary purpose of using restriction enzymes in gel electrophoresis?

• It allows the strands of DNA to be cut into various lengths for testing

• It restricts the number of base pairs that can be tested in a sample

• It makes the testing simpler by moving the strands into the gel faster

• It charges the DNA strands

Which DNA fragment bands move faster and farther in a gel electrophoresis? 

• there is no way to tell 

• the largest ones

• the smallest ones

• none of these

Which suspect is linked to the crime scene by this DNA analysis

• Suspect A

• Suspect C

• Suspect D

• Suspect B

Tags

NGSS.HS-LS3-1

What is the role of the buffer in gel electrophoresis?

• To conduct the electric current

• To stain the DNA fragments

• To filter DNA based on size

• To create indentations in the gel

Which suspect is likely guilty?

• Suspect #1

• Suspect #2

• Suspect #3

Tags

NGSS.HS-LS3-1

NGSS.HS-LS3-2

Known for using small, trace amounts of DNA and generating multiple identical copies.

• PCR (Polymerase Chain Reaction)

• Use Restriction Enzymes

• Gel Electrophoresis

The DNA is then mixed with ________, which cut the DNA in specific places that will result in different sized fragments.

• Gel Electrophoresis

• PCR (Polymerase Chain Reaction)

• Restriction Enzymes

Begins at a high temperature of 94C for a minute. Afterwards, the DNA strand is untwisted and unzipped in order to expose the base pairs of sequences. The DNA is now broken into two separate strands.

• Elongation by DNA Polymerase

• Primer Annealing

• Denaturation