Mi 1.4

What is the significance of the recognition sequence for a restriction enzyme?

• It determines the enzyme's ability to cut DNA.

• It specifies the type of DNA base pairs it can recognize.

• It influences the enzyme's catalytic activity.

• It regulates the enzyme's expression in the cell.

Which of the following is an application of restriction enzymes in molecular biology?

• Sequencing DNA fragments

• Polymerase chain reaction (PCR)

• Transcription of mRNA from DNA templates

• Generating recombinant DNA molecules

Why was it important to not disrupt the gene for antibacterial resistance?

• We needed this to grow it in an antibiotic medium to see if it took up the plasmid

• Since this is a bacteria, we needed to give the patient an antibiotic so the bacteria wouldn’t infect their cells

• The antibiotic gene works with the gene of origin to determine which one gets transcribed and translated

• It was not important as long as it didn’t get cut into more than two pieces

Tags

NGSS.HS-LS4-4

Which enzyme acts like glue to joint the complementary base pairs of the cut DNA on the plasmid?

• gyrase

• polymerase

• ligase

• helicase

Why are plasmids so important to vaccine production?

• plasmids can be used as a vector to carry genes of interest into a bacteria

• plasmids are easy to cut and paste and have a nearly 100% effective rate

• plasmids are stationary inside a bacterium and can’t move

• plasmids are involved in mitosis and thus involved in producing new cells

What is important to scientists when producing a new vaccine?

• the safety of the vaccine

• the easy of delivery

• its overall effectiveness over time

• all of these are important factors

Tags

NGSS.HS-ETS1-3

NGSS.HS-ETS1-4

Generally, there are two types of vaccines: attenuated and inactivated. What does “attenuated” mean in this context?

• live and strong

• live and weak

• killed and strong

• killed and weak

What is the significance of the recognition sequence for a restriction enzyme?

• It determines the enzyme's ability to cut DNA.

• It specifies the type of DNA base pairs it can recognize.

• It influences the enzyme's catalytic activity.

• It regulates the enzyme's expression in the cell.

Which of the following is an application of restriction enzymes in molecular biology?

• Sequencing DNA fragments

• Polymerase chain reaction (PCR)

• Transcription of mRNA from DNA templates

• Generating recombinant DNA molecules

Why was it important to not disrupt the gene for antibacterial resistance?

• We needed this to grow it in an antibiotic medium to see if it took up the plasmid

• Since this is a bacteria, we needed to give the patient an antibiotic so the bacteria wouldn’t infect their cells

• The antibiotic gene works with the gene of origin to determine which one gets transcribed and translated

• It was not important as long as it didn’t get cut into more than two pieces