PCR Quiz

PCR is useful for all of the following reasons except:

• targeting a specific region in a huge genome

• identifying proteins with site specific mutant amino acids

• identifying size differences between individuals at a particular locus

• making many copies of a particular gene segment

When using PCR for identifying purposes,

• highly conserved regions of the genome should be targeted

• highly variable regions of the genome should be targeted

• any area on the genome would work well

• making many copies of a particular gene segment

What is the most commonly used DNA polymerase in PCR

• synthetic DNA polymerase

• polymerase from a thermophilic bacteria

• E. coli polymerase

• human DNA polymerase

Which of the following is not a regular use of PCR

• Forensics

• Kinship/paternity testing

• Body identification

• Protein identification

Discuss the factors to consider when designing PCR primers.

• Primer length, GC content, melting temperature, specificity, and avoiding self-complementarity

• Weather conditions, time of day, and personal preferences

• Number of test tubes, type of pipette, and lab location

• Primer color, font size, and spacing

Describe the process of PCR amplification.

• PCR amplification is a technique used to identify a specific DNA segment

• PCR amplification is a technique used to reduce the number of DNA copies

• PCR amplification is a technique used to make multiple copies of a specific DNA segment.

• PCR amplification is a technique used to make multiple copies of RNA

What is the purpose of PCR?

• To produce millions of copies of DNA.

• To produce millions of copies of a specific region of DNA

• To add nucleotides to a DNA sequence

• To watch polymerase work.

Explain the steps involved in the Polymerase Chain Reaction process.

• Folding, Splicing, and Packaging

• Denaturation, Annealing, and Extension

• Replication, Transcription, and Translation

• Mixing, Heating, and Cooling

What is the significance of the annealing step in the PCR process?

• It helps in the separation of the DNA strands.

• It allows the primers to bind to the DNA template.

• It helps in the synthesis of new DNA strands.

• It helps in the extraction of DNA from the sample.

At what temperature does the Denature step of PCR occur?

• 94-96° C

• 50-65° C

• 72° C

• Any temperature