MI 3.4

DNA has a ___________ charge and will migrate through a gel towards the ___________ electrode.

• positive, negative

• positive, positive

• negative, positive

• negative, negative

Larger DNA fragments move through a gel _____________ smaller DNA fragments.

• faster than

• at the same rate as

• slower than

What do dideoxynucleotides do when added to a growing DNA strand during Cycle Sequencing?

• They stop the elongation of that strand.

• That double the length of that strand.

• They shorten the length of the strand.

• They denature the strand.

Which of the following base pairs is matched correctly?

• Guanine and Thymine

• Cytosine and Adenine

• Adenine and Thymine

• Thymine and Cytosine

How is DNA synthesis in PCR and Cycle Sequencing DIFFERENT?

• Only PCR uses deoxynucleotides.

• Cycle Sequencing generates DNA products of different lengths.

• Only PCR analyzes DNA products using gel electrophoresis.

• Only Cycle Sequencing uses dideoxynucleotides.

Place the steps of DNA Sequencing below into their correct order.

• A, B, C, D

• B, C, A, D

• B, A, C, D

• D, B, C, A

Write the correct sequence that matches this chromatogram.

Show all your work on your answer document. (Average speed = total distance / total time)

• 6.25 km/min

• 21 m/s

• 0.16 km/min

• 100 m/s

Tags

NGSS.MS-PS3-1

Show all your work on your answer document. (Average speed = total distance / total time)

• 1.6 km/min

• 1.6 m/s

• 0.625 km/min

• 0.625 m/s

Tags

NGSS.MS-PS3-1

Show all your work on your answer document.

• 55 mi/hr

• 0.018 mi/hr

• 0.018 hr/mi

• 220 mi/hr