Effect of Temperature and pH on Enzymatic Activity

What happens to enzymes at high temperatures?

• Enzymes become inactive.

• Enzymes denature

• Enzymes break down.

• Enzymes become more active.

How does temperature affect enzyme-substrate interactions?

• Temperature has no effect on enzyme-substrate interactions.

• Temperature increases the concentration of enzymes in the solution.

• Temperature affects enzyme-substrate interactions by altering the kinetic energy of the molecules.

• Temperature decreases the rate of enzyme-substrate interactions.

At what temperature does enzyme activity start to decrease?

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NGSS.HS-PS1-5

At what temperature does enzyme activity start to increase rapidly?

At what temperature does enzyme activity at optimal speed?

What is the process called when enzymes lose their structure and function at high temperatures?

• denaturation

• reduction

• activation

• inhibition

What are some factors that can affect the thermal stability of enzymes?

• Light intensity, pressure, humidity

• Salt concentration, enzyme concentration, reaction time

• Enzyme size, reaction volume, stirring speed

• pH, temperature, substrate concentration, inhibitors or activators

How does temperature affect the rate of enzyme-substrate reactions?

• Temperature affects the rate of enzyme-substrate reactions by increasing it up to an optimal temperature and then decreasing it beyond that point.

• Decreasing temperature always decreases the rate of enzyme-substrate reactions.

• Increasing temperature always increases the rate of enzyme-substrate reactions.

• Temperature has no effect on the rate of enzyme-substrate reactions.

What is the optimal pH range for most human enzymes?

• A) 1-2

• B) 4-5

• C) 6-8

• D) 9-11

How does a decrease in temperature generally affect enzyme activity?

• A) Increases activity

• B) Decreases activity

• C) No effect

• D) Denatures the enzyme