Applied DNA

A technique for making millions of copies of a specific region of DNA.

• DNA ligase

• gel electrophoresis

• restriction enzymes

• polymerase chain reaction

What happens during the Anneal step of PCR?

• Primers are created.

• Primers copy the new DNA strand

• The primers attach to the target DNA region.

• Primers sequence DNA

What is the function of a primer?

• To identify the particular region of DNA to be copied by PCR.

• To create DNA nucleotides

• To copy DNA.

• To maintain the temperature of the PCR reaction.

A method used to rapidly make multiple copies of a specific segment of DNA.

• PCR (Polymerse Chain Reaction)

• Electrophoresis

• DNA Probe

This is the last step of PCR and it requires the temperature to increase to 72C for one minute. Here, complementary strands will be produced from the corresponding base pairs, reforming the DNA into two new strands.

• Elongation by DNA Polymerase

• Primer Annealing

• Denaturation

There are multiple male suspects involved in a case. Which of the following analysis techniques would be most helpful in differentiating the men from one another ?

• Y-Marker Analysis

• SNP Analysis

• Amelogenin Gene Analysis

• Mitochondrial DNA Analysis

Tags

SFS3

Which is the primary purpose of using restriction enzymes before gel electrophoresis?

• It cuts the DNA strands in a set location for analysis.

• It restricts the number of base pairs that can be tested in a sample

• It makes the testing simpler by moving the strands into the gel faster

• It charges the DNA strands

Which part(s) of a hair can be analyzed for DNA?

• root

• cuticle

• medulla

• cortex

DNA can be used for ...

• determining paternity

• identifying human remains

• identifying a suspect

• All of these

Tags

SFS3

Why are restriction enzymes used when making a DNA fingerprint using STRs?

• They are used to determine the size and shape of DNA fragments.

• They are used in order to allow us to determine if a specific family or family member is related to the crime based on their DNA fragments.

• STR bands are units of bases that are typically two to five bases long and repeat multiple times. Restriction enzymes cut these bands since they match the enzyme, making them easily recognisable.