GFP Bacterial Transformation

The process of getting a bacteria cell to uptake DNA plasmids using the calcium chloride and heat shock method is known as

• transduction

• transformation

• conjugation

• translation

This element is required to turn on the GFP gene

• glucose

• sucrose

• IPTG

• fructose

How do you calculate if your transformation lab was a success?

• pearson coefficient

• transformation efficiency

• average mean

• transformation median

If you were successful at purifying to GFP, you will see

• green glowing bacterial colonies

• bright green drops falling from the column

• several glowing green bands on the gel

• one band on the gel

In the GFP lab, why did we grow -pGLO on LB?

• To prove that the bacteria didn't alreadt glo

• To prove the bacteria needed ampicillin to grow

• To prove that bacteria had no IPTG in it

• To prove that bacteria can undergo conjugation

The plasmids scientists use in a lab are MOST COMMONLY found in

• plants

• animals

• archaea

• bacteria

Why are cells incubated at 42^o C?

• At 42^o C, bacterial cells begin releasing DNA for other cells to take up

• This is a good recovery temperature after heat shock

• At 42^o C, the membranes of bacterial cells begin to degrade, creating pores through which DNA can enter the cell

Arabinose is used in the plates to

• isolate and select for only the bacteria that have been successfully transformed

• give bacteria energy to grow

• act as a trigger to turn on the expression of the gene of interest

• give the bacteria a new trait (such as fluorescence or insulin production)

What is the primary purpose of using GFP in bacterial transformation experiments?

• To make bacteria resistant to antibiotics

• To visualize gene expression

• To increase bacterial growth rate

• To alter bacterial DNA sequence

Which of the following is a common method to introduce GFP plasmids into bacterial cells?

• Electroporation

• Conjugation

• Transduction

• Binary fission