Unit 1 Insta-Review

Which direction is DNA synthesized?

• 3’ to 5’

• 5’ to 3’

• Right to Left

• Left to Right

Which DNA tech separates fragments by size/charge?

• Gel electrophoresis

• PCR

• Sequencing

• Transformation

What cuts the DNA prior to running gel electrophoresis?

• DNA polymerase

• Helicase

• Ligase

• Restriction enzyme

Minimum restriction sites for ECO on plasmid to get this?

• 1

• 2

• 3

• 4

Which DNA tech is responsible for multiple copies of a gene?

• Gel electrophoresis

• PCR

• Sequencing

• Transformation

Plate has non transformed bacteria & no antibiotics

• I

• II

• III

• IV

If plasmid also had the gene to synthesize insulin... Which plate has the highest % of insulin producing bacteria?

• I

• II

• III

• IV

Tags

NGSS.HS-LS3-2

DNA is ___ because of the ____.

• Negatively charged; hydroxyl group

• Negatively charged;  phosphate group

• Positively charged;  hydroxyl group

• Positively charged;  phosphate group

What does a thicker band represent in a gel?

• There’s more DNA material present in that band

• There’s more glucose available in that band

• There’s more mutation in the DNA fragment present

• There’s more radioactive material present in that band

What is used to cut the DNA into fragments for the gel?

• Ligase from eukaryotes

• Ligase from prokaryotes

• Restriction enzymes from eukaryotes

• Restriction enzymes from prokaryotes