Restriction Enzymes and Gel Electrophoresis

EcoRI, HaeIII, HindIII are examples of...

• nitrogen bases

• digestion sites

• DNA strands

• restriction enzymes

In preparation for an electrophoresis procedure, enzymes are added to DNA in order to

• convert the DNA into gel

• cut the DNA into fragments

• change the color of the DNA

• produce longer sections of DNA

How many restriction sites appear in the picture?

• 1

• 2

• 3

• 4

After DNA has been cut into pieces by restriction enzymes, it is considered to be...

• snipped

• amplified

• digested

• Cut up

The location where restriction enzymes cut DNA is called a..

• nucleotide

• recognition site

• phosphate

• molecular clevage site

This laboratory procedure is known as

• CLONING

• gel electrophoresis

• chromatography

• use of a dichotomous key

A student performed a gel electrophoresis experiment. The results are represented in the diagram

below. Compared to the fragments at the top of the gel, the fragments at the lower end are

• larger, and move slower

• larger, and move faster

• smaller, and move faster

• smaller, and move slower

Restriction Enzymes . . . 

• cut DNA at precise locations

• help bacteria to pick up DNA

• move DNA through a gel

• copy DNA

Restriction Enzymes come from . . . 

• viruses

• bacteria

• goats

• spiders

An individual’s code determines the number of times the restriction enzymes will cut and the number and size of DNA pieces that will result.

• True

• False