Biotechnology 9.3. HW

In open-column chromatography, what is the main advantage of using small columns?

• Faster separation

• Easier setup

• Better quantitation

• Larger sample volume

Fast-Performance Liquid Chromatography (FPLC) was developed in the 1970s to address the need for ________.

• Faster separation

• Larger columns

• More complex equipment

• Slower processes

What is the main purpose of using a UV spectrophotometer in Fast-Performance Liquid Chromatography (FPLC)?

• To separate the sample into fractions

• To analyze the sample for ID and concentration

• To push the sample through the resin

• To equilibrate the column with buffers.

In Fast-Performance Liquid Chromatography (FPLC), the sample is pushed through the resin in the column under pressure. What component is responsible for this action?

• A) UV spectrophotometer

• B) Buffer reservoir

• C) Peristaltic pump

• D) Fraction collector

In Fast-Performance Liquid Chromatography (FPLC), the sample is analyzed by a UV spec for ID and concentration. Fill in the blank: The sample is separated into _______ automatically.

• columns

• fractions

• buffers

• resins

What is the main purpose of high-performance liquid chromatography (HPLC)?

• To separate and purify samples

• To heat samples

• To cool samples

• To mix samples.

In column chromatography, which type of resin would you use to separate molecules of 60 kDa from 250 kDa proteins?

• Sephacryl-100

• Sephacryl-250

• Sephacryl-300

What is the role of Mg2+ and Ca2+ ions in ion-exchange water softeners?

• They attract Na+ ions

• They knock Na+ ions off ion-exchange resin

• They dissolve in water

• They form a precipitate.

In ion-exchange chromatography, an elution buffer with a high salt concentration can be used to ______.

• dissolve the sample

• knock a bound protein off the charged beads

• attract negatively charged molecules

• stabilize the pH.

What is the purpose of using a dialysis bag in a buffer exchange process?

• To increase the concentration of the sample

• To allow the exchange of old and new buffers

• To separate proteins from the buffer

• To measure the volume of the buffer.