High Performance Liquid Chromatography Quiz

Explain the principles of liquid chromatography

Draw and label the schematic diagram of the components in HPLC

Able to explain the functions of each component in HPLC

Discuss the difference between each component in terms of parts and functions

Interpret analytical information from chromatographic data for HPLC

Recognize problems arising from resultant chromatograms and suggest probable solutions

What is the function of the mobile phase in HPLC?

• To separate the components of a mixture

• To inject the sample

• To detect the components

• To maintain stable flow

What are the types of pumps used in HPLC?

• Reciprocating pumps

• Displacement pumps

• Pneumatic pumps

• All of the above

What is the purpose of a guard column?

• To increase the lifetime of an analytical column

• To decrease pressure drop

• To prevent clogging

• All of the above

What is the particle size of pellicular packing?

• 30-40 μm

• 3-10 μm

• 5-10 μm

• 10-30 μm