Maxam-Gilbert Sequencing and Blotting Quiz

Which of the following is the primary principle behind Maxam-Gilbert sequencing?

• Enzymatic DNA synthesis

• Chemical modification and cleavage of DNA

• Fluorescent dye incorporation

• Single-molecule sequencing

What chemical is used in Maxam-Gilbert sequencing to methylate guanine (G) only?

• Formic acid

• Hydrazine

• Dimethyl sulfate (DMS)

• Piperidine

What is a key disadvantage of Maxam-Gilbert sequencing?

• Requires DNA polymerase

• High throughput

• Use of toxic chemicals

• Less accuracy in short sequences

What is the main purpose of Southern blotting?

• To detect specific RNA sequences

• To amplify DNA fragments

• To determine the identity, size, and abundance of DNA sequences

• To separate proteins based on their size

In Southern blotting, which step ensures the transfer of DNA from gel to a nylon membrane?

• Denaturation

• Blotting

• Hybridization

• PCR amplification

What is the purpose of restriction enzymes in Southern blotting?

• To break down RNA molecules

• To cut DNA at specific recognition sites

• To denature proteins

• To hybridize the probe

Who developed the Polymerase Chain Reaction (PCR) technique?

• Kary Mullis

• Edward Southern

• Frederick Sanger

• Walter Gilbert

What is the role of primers in PCR?

• Act as templates for DNA synthesis

• Bind to complementary sequences to initiate replication

• Break the DNA strand

• Replace nucleotides

What is the primary goal of DNA sequencing?

• To create synthetic DNA

• To determine the exact order of nucleotides in a DNA molecule

• To clone genes

• To modify genetic material

In Maxam-Gilbert sequencing, hydrazine + salt is used to cleave:

• Thymine

• Guanine

• Cytosine

• Adenine