Molecular Diagnostics, Biosensor Development, and Microfludics

How can you increase the sensitivity in molecular diagnostics?

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Intercalating dyes bind to single stranded DNA.

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Short single stranded DNA sequences that recognise conserved regions in an organism's nucleic acid are called?

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What is PCR?

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What are the 3 stages in PCR and what conditions are required for them?

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Dyes which aid the visualization of DNA amplification are called?

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Apart from dyes, how can you determine the success of nucleic acid amplification?

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Which enzyme is responsible for DNA amplification?

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I am interested in detecting the virus responsible for Dengue hemorrhagic fever. It is an RNA virus. Describe the steps l need to detect this virus using molecular diagnostic principles.

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What are the steps involved in DNA extraction?

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