Molecular Diagnostics, Biosensor Development, and Microfludics

How can you increase the sensitivity in molecular diagnostics?

Intercalating dyes bind to single stranded DNA.

• True

• False

Short single stranded DNA sequences that recognise conserved regions in an organism's nucleic acid are called?

What is PCR?

What are the 3 stages in PCR and what conditions are required for them?

Dyes which aid the visualization of DNA amplification are called?

Apart from dyes, how can you determine the success of nucleic acid amplification?

Which enzyme is responsible for DNA amplification?

I am interested in detecting the virus responsible for Dengue hemorrhagic fever. It is an RNA virus. Describe the steps l need to detect this virus using molecular diagnostic principles.

What are the steps involved in DNA extraction?