DNA Fingerprinting

Why is DNA Fingerprinting called a 'fingerprint'?

• All of a person's DNA is found in their fingers
• A person's DNA Fingerprint looks the same as a person's finger fingerprint
• Everyone's finger fingerprint is unique, just as everyone's DNA Fingerprint is unique
• The tool used to make a DNA Fingerprint is called 'The DNA Fingerprint Machine'

What is the first step to making a DNA Fingerprint?

• Make millions of copies of the DNA
• Cut the DNA into different sized pieces
• Separate the DNA pieces by size
• Compare DNA fingerprints, find a match

What is the second step to making a DNA Fingerprint?

• Make millions of copies of the DNA
• Cut the DNA into different sized pieces
• Separate the DNA pieces by size
• Compare DNA fingerprints, find a match

What is the third step to making a DNA Fingerprint?

• Make millions of copies of the DNA
• Cut the DNA into different sized pieces
• Separate the DNA pieces by size
• Compare DNA fingerprints, find a match

Three tools are used to make a DNA fingerprint - which one is like scissors that cuts DNA at a particular sequence?

• PCR Machine
• Restriction Enzyme
• DNA Ligase
• Gel Electrophoresis machine

Three tools are used to make a DNA fingerprint - which one is like a biological copy machine, making thousands of copies of each piece of DNA?

• PCR Machine
• Restriction Enzyme
• DNA Ligase
• Gel Electrophoresis machine

Three tools are used to make a DNA fingerprint - which one separates pieces of DNA by size?

• PCR Machine
• Restriction Enzyme
• DNA Ligase
• Gel Electrophoresis machine

Why do DNA pieces move from the top of the Gel towards the bottom?

• there is an electric charge in the gel, (+) at the top, which repels the (+) DNA molecules
• there is an electric charge in the gel, (-) at the top, which repels the (+) DNA molecules
• there is an electric charge in the gel, (-) at the top, which repels the (-) DNA molecules
• there is an electric charge in the gel, (+) at the top, which repels the (-) DNA molecules

During the Cut step, why would your DNA be cut into different sized pieces than your neighbor's DNA?

• A different restriction enzyme is used
• Your DNA has a different sequence than your neighbor's
• Restriction enzymes cut randomly, no two Fingerprints are ever the same
• DNA samples are heated to different temperatures, causing DNA to break into different sized pieces

When DNA segments are being separated by size,

• the small strands stay near the top, the big ones move to the bottom
• the big strands stay near the top, the little ones move to the bottom
• the small strands move to the middle, and the big ones move to the sides
• the big strands move to the middle, and the small ones move to the sides