Chapter 3 - DNA Sequencing slides 28-49

What does SMRT sequencing use to copy DNA?
A) RNA polymerase
B) DNA polymerase
C) Restriction enzymes
D) CRISPR

• A

• B

• C

• D

What is a major advantage of SMRT sequencing?
A) Short read lengths
B) Fast sequencing and long reads
C) No need for library preparation
D) 100% accuracy in one run

• A

• B

• C

• D

What is the error rate per run?
A) 1%
B) 5%
C) 10-15%
D) 50%

• A

• B

• C

• D

How much does an SMRT sequencing machine cost?
A) $4,000
B) $90,000
C) $900,000
D) $9,000,000

• A

• B

• C

• D

What does Oxford Nanopore sequencing use to detect DNA sequences?
A) Fluorescent labels
B) Electric current changes
C) Polymerase enzymes
D) Mass spectrometry

• A

• B

• C

• D

What is one major advantage of ONT sequencing?
A) It uses fluorescent signals for detection
B) It requires a lot of sample preparation
C) It does not need DNA amplification or labeling
D) It can only read short DNA fragments

• A

• B

• C

• D

How does ONT sequencing work?
A) DNA polymerase copies the DNA into new strands
B) DNA fragments are passed through a nanopore, and current changes are measured
C) DNA is broken into short pieces and detected by lasers
D) CRISPR is used to edit and read the DNA

• A

• B

• C

• D

What is one challenge of ONT sequencing?
A) It cannot read long DNA sequences
B) It is too expensive to use
C) Accuracy and parallelization still need improvement
D) It requires complex fluorescent dyes

• A

• B

• C

• D

What is the potential cost of sequencing a genome with ONT?
A) $10
B) $100
C) $10,000
D) $1,000,000

• A

• B

• C

• D

What does a FASTQ file store?
A) Only DNA sequences
B) DNA sequences and quality scores
C) Protein sequences
D) Only quality scores

• A

• B

• C

• D