Biotechnology I Final Exam Review

This technique ensures that sterile conditions are maintained in the lab.

• Aseptic Technique

• Contamination Method

• Infection Procedure

• Non-Sterile Technique

Answer explanation

The Aseptic Technique is specifically designed to maintain sterile conditions in the lab, preventing contamination and ensuring the integrity of experiments. Other options do not focus on maintaining sterility.

This term refers to a method of amplifying small DNA samples.

• PCR (Polymerase Chain Reaction)

• Gel Electrophoresis

• Southern Blotting

• DNA Sequencing

Answer explanation

PCR (Polymerase Chain Reaction) is a technique specifically designed to amplify small DNA samples, making it easier to study and analyze them. Other options like Gel Electrophoresis and Southern Blotting are used for different purposes.

This biotechnology branch focuses on healthcare and medicine.

• Red Biotechnology

• Green Biotechnology

• Blue Biotechnology

• White Biotechnology

Answer explanation

Red Biotechnology is the branch of biotechnology that focuses specifically on healthcare and medicine, including the development of pharmaceuticals and medical treatments.

This document explains unexpected events or errors in a lab process.

• Deviation Report

• Lab Manual

• Safety Guidelines

• Experiment Protocol

Answer explanation

A Deviation Report documents unexpected events or errors in lab processes, making it the correct choice. The Lab Manual, Safety Guidelines, and Experiment Protocol do not specifically address errors.

This tool is used to measure and transfer very small liquid volumes.

• Micropipette

• Graduated Cylinder

• Beaker

• Burette

Answer explanation

A micropipette is specifically designed to measure and transfer very small liquid volumes with high precision, making it the correct choice. Other options like graduated cylinders and beakers are not suitable for such small measurements.

Contamination occurred while transferring bacterial cultures. What key technique may have been missed?

• Aseptic technique

• Proper labeling

• Temperature control

• Time management

Answer explanation

Aseptic technique is crucial in microbiology to prevent contamination during culture transfers. If this technique is not properly followed, unwanted microorganisms can easily contaminate the cultures.

You are a lab technician investigating a contamination issue in your lab. Follow the steps below to identify what went wrong and fix the issue. Which of the following steps would you take to improve aseptic technique?

• Wear gloves and sterilize equipment before use

• Ignore minor spills and focus on major ones

• Use the same equipment for different experiments without cleaning

• Allow unauthorized personnel into the lab

Answer explanation

Wearing gloves and sterilizing equipment are essential for maintaining aseptic technique, preventing contamination. Ignoring spills, reusing unclean equipment, and allowing unauthorized personnel increase contamination risks.

You are a lab technician investigating a contamination issue in your lab. Follow the steps below to identify what went wrong and fix the issue. Identify one type of documentation you would create to record an incident.

• Incident Report

• Lab Notebook

• Safety Data Sheet

• Maintenance Log

Answer explanation

An Incident Report is essential for documenting contamination issues, detailing what occurred, how it was discovered, and actions taken. This helps in identifying the cause and preventing future incidents.

What is the purpose of a centrifuge in DNA extraction?

• To separate DNA from other cellular components

• To heat the DNA sample

• To measure the concentration of DNA

• To cut DNA into smaller fragments

Answer explanation

The purpose of a centrifuge in DNA extraction is to separate DNA from other cellular components. It uses centrifugal force to spin samples, allowing denser materials to settle at the bottom, isolating the DNA.

The enzyme used to cut DNA at specific sequences for cloning is:

• DNA polymerase

• Ligase

• Restriction enzyme

• Helicase

Answer explanation

The correct answer is 'Restriction enzyme' because these enzymes specifically cut DNA at designated sequences, which is essential for cloning. Other options like DNA polymerase and ligase serve different functions in DNA manipulation.