Quiz on Polymerase Chain Reaction (PCR)

What is the purpose of the polymerase chain reaction (PCR)?

• To create large quantities of identical DNA from trace samples

• To break down DNA into smaller fragments

• To sequence the entire genome

• To alter the genetic code of an organism

Which of the following is a potential application of PCR?

• Analyzing crime scene samples

• Creating new species

• Developing new antibiotics

• Producing synthetic proteins

Which component is used to initiate the PCR process?

• Primers

• Nucleotides

• Mix Buffer

• PCR Tube

What is the role of reverse primers in PCR?

• Bind to the start codon at the 3’ end of the template strand.

• Bind to the stop codon at the 3’ end of the coding strand.

• Bind to the start codon at the 5’ end of the coding strand.

• Bind to the stop codon at the 5’ end of the template strand.

What is the process called where DNA replication occurs at an exponential rate?

• Linear Replication

• Exponential Replication

• Circular Replication

• Random Replication

How many copies are produced after 2 cycles of exponential DNA replication?

• 2 copies

• 4 copies

• 8 copies

• 16 copies

What is the significance of the red wavy line in the diagram?

• It represents RNA.

• It represents the target DNA.

• It represents proteins.

• It represents lipids.

What is the first step in the PCR process?

• Denaturation at 90-95°C

• Annealing at 50-55°C

• Elongation at 72°C

• Replication at 60°C

At what temperature does the annealing step of PCR occur?

• 90-95°C

• 50-55°C

• 72°C

• 60-65°C

During which step of PCR are the DNA strands separated?

• Denaturation

• Annealing

• Elongation

• Replication