Gene Technology Quiz

What is another name for restriction endonucleases?

• DNA ligases

• Restriction enzymes

• RNA polymerases

• DNA helicases

What molecules are involved in the bonds cleaved by endonucleases?

• Deoxyribose sugar and phosphate

• Ribose sugar and nitrogenous base

• Amino acids and proteins

• Lipids and carbohydrates

What is the main function of the phosphodiester bond in DNA?

• To hold the DNA strands together

• To connect deoxyribose sugar and phosphate

• To code for proteins

• To initiate DNA replication

What is the primary action of EcoR1 on DNA?

• It synthesizes DNA.

• It cuts DNA at specific sequences.

• It repairs DNA.

• It unwinds DNA.

What is the role of an endonuclease in DNA processing?

• It joins DNA fragments together.

• It cuts the double-stranded DNA molecule at a specific recognition site.

• It replicates DNA strands.

• It synthesizes new DNA strands.

What type of bond is formed when sticky ends join together?

• Hydrogen bond

• Ionic bond

• Covalent bond

• Metallic bond

Why are blunt ends considered non-specific?

• They can pair with any DNA strand

• They cannot pair with a complementary DNA strand

• They are too long

• They are too short

Which enzyme is responsible for joining DNA fragments?

• DNA polymerase

• DNA ligase

• RNA polymerase

• Helicase

What is the significance of phosphodiester bonds in DNA?

• They hold the DNA double helix together

• They connect nucleotides in a DNA strand

• They form the backbone of RNA

• They are involved in protein synthesis

Diagram showing the process of DNA replication with primers and polymerase.

• Diagram showing the process of DNA transcription with ribosomes and mRNA.

• Diagram showing the process of protein synthesis with tRNA and ribosomes.

• Diagram showing the process of cell division with centrioles and spindle fibers.

• Diagram showing the process of DNA replication with primers and polymerase.