Expt 6_Bio Lab

Enzymes are _____

• Nucleic acids

• Proteins

• Triglycerides

• All of the above

Which of the following are the advantages of enzymes as biocatalyst?

• Substrate specificity

• Selectivity

• Benign reaction conditions

• Activity at higher temperature

Rate of enzyme catalysed reaction depends on _____

• Substrate concentration

• Enzyme concentration

• pH

• Temperature

Which enzyme are we using today?

• Hydrolase

• Phosphatase

• Polymerase

• Ligase

What is the source of phosphatase enzyme?

• Onion peal

• Potato tubers

• Spinach leaves

• Mung bean seedlings

How are we going to extract the enzyme from Mung seedlings?

• Grinding into a paste --> Centrifugation

• Boiling in water --> Centrifugation

• Adding directly

• Draining the water through filtration

Which of the following reaction are we going to carry out today?

• Glucose --> Fructose

• Phenolphthalein phosphate --> Phenolphthalein

• Glucose phosphate --> Glucose

• DNA --> RNA

What temperature will we carry out the reaction?

• Room temperature

• 30 C

• 37 C

• 50 C

What is the variable here?

• Substrate Concentration

• Enzyme Concentration

• Time

• Temperature

How will we detect the product?

• Centrifugation to remove the substrate

• The product is colored at the reaction condition

• Boiling the mixture to develop the color

• Changing the pH to alkaline using NaCO3 to develop color