Topic 12: Enzyme Kinetics

In a Lineweaver–Burk plot, what does the y-intercept represent?

Which of the following is a characteristic of competitive enzyme inhibition?

• The inhibitor binds to the enzyme-substrate complex

• The inhibitor binds to the active site of the enzyme

• The inhibitor binds to an allosteric site

• The inhibitor permanently deactivates the enzyme

Which type of inhibitor can be overcome by increasing substrate concentration?

• Noncompetitive inhibitor

• Irreversible inhibitor

• Competitive inhibitor

• Allosteric inhibitor

What is the primary effect of an irreversible inhibitor on an enzyme?

• It temporarily reduces enzyme activity

• It permanently inactivates the enzyme

• It increases enzyme activity

• It changes the enzyme's substrate specificity

• The maximum reaction rate

• The substrate concentration at half-maximum velocity

• The enzyme concentration

• The inhibitor concentration

Which of the following is true about noncompetitive inhibition?

• The inhibitor competes with the substrate for the active site

• The inhibitor binds only to the enzyme-substrate complex

• The inhibitor can bind to both the enzyme and the enzyme-substrate complex

What is the effect of a noncompetitive inhibitor on the Lineweaver–Burk plot?

• It increases the slope and changes the y-intercept

• It decreases the slope and changes the x-intercept

• It increases the slope without changing the x-intercept

• It decreases the slope without changing the y-intercept

Which of the following statements is true about enzyme kinetics?

• Enzyme kinetics is independent of substrate concentration

• Enzyme kinetics can be described by the Michaelis–Menten equation

• Enzyme kinetics is only applicable to irreversible reactions

• Enzyme kinetics is not affected by enzyme concentration