Unit 6 Ch 18

Which of the following is used to cut DNA at specific base pair sequences?

• DNA ligase

• gel electrophoresis

• polymerase chain reaction

• restriction enzymes

A forensic scientist recovers a very small amount of evidence at a crime scene. The scientist would like to amplify the number of copies of DNA in the evidence sample. Which of the following techniques should the scientist use?

• bacterial transformation

• CRISPR-Cas9

• gel electrophoresis

• polymerase chain reaction

A forensic scientist cuts DNA from a crime scene and DNA from a suspect with the same enzyme. Which tool should the scientist use to separate the DNA fragments according to their size?

• bacterial transformation

• CRISPR-Cas9

• gel electrophoresis

• polymerase chain reaction

What should the scientist use to add the DNA code for human growth hormone to a plasmid?

• bacterial transformation

• DNA ligase

• gel electrophoresis

• polymerase chain reaction

The scientist has successfully created a plasmid that contains the DNA code for the human growth hormone gene. Which technique should the scientist use to insert the plasmid into a cell?

• bacterial transformation

• CRISPR-Cas9

• gel electrophoresis

• polymerase chain reaction

Which of the following tools would be most useful in amplifying the number of copies of the HTT gene so that more DNA would be available for analysis?

• CRISPR-Cas9

• gel electrophoresis

• polymerase chain reaction

• restriction enzymes

Which tool would be most useful for estimating the size of the HTT gene isolated?

• DNA ligase

• gel electrophoresis

• polymerase chain reaction

• restriction enzymes

A transgenic mouse has the HTT gene. A scientist wants to investigate whether inserting a stop codon into the HTT gene might prevent the mouse from developing the disease. Which of the following tools would be most useful in inserting a stop codon into the HTT gene?

• CRISPR-Cas9

• gel electrophoresis

• polymerase chain reaction

• restriction enzymes

What property of DNA causes it to move toward the positive electrode in gel electrophoresis?

• the nitrogen atoms in the nitrogenous bases

• the hydrogen bonds that form between the bases in the two strands of the double helix

• the charges on the oxygen atoms in the deoxyribose sugars

• the charges on the phosphates in the backbone of the helix

Why is a heat-stable DNA polymerase required for the polymerase chain reaction (PCR)?

• Heat-stable DNA polymerases add DNA nucleotides at a faster rate than other DNA polymerases.

• The high temperatures that are required for each cycle of PCR to separate the DNA strands would denature DNA polymerases that are not heat stable.

• Heat-stable DNA polymerases anneal more readily to the PCR primers.

• Heat-stable DNA polymerases are more accurate than other DNA polymerases.