Quiz on ECM and Tissue Decellularization

What is the primary function of the extracellular matrix (ECM)?

• To provide energy to cells

• To store genetic information

• To serve as a passive scaffold

• To provide structural and biochemical support to surrounding cells

Which of the following is NOT a major component of the ECM?

• Collagens

• Elastins

• Nucleic acids

• Proteoglycans

What is the goal of tissue decellularization?

• To increase tissue size

• To introduce synthetic materials

• To enhance cellular growth

• To remove cellular components while preserving ECM

Which method is commonly used to remove cellular material from tissues?

• Chemical synthesis

• Electrolysis

• Freeze-thaw cycles

• Thermal expansion

What is a significant challenge in the decellularization process?

• Maintaining cellular integrity

• Achieving complete cell removal without ECM damage

• Increasing the size of the tissue

• Enhancing the color of the tissue

Which of the following is a common chemical agent used in decellularization?

• Sodium chloride

• Sodium dodecyl sulfate (SDS)

• Hydrogen peroxide

• Ethanol

What is the purpose of using proteolytic enzymes in decellularization?

• To increase the size of the ECM

• To preserve nucleic acids

• To cleave cellular proteins and aid in cell removal

• To enhance the color of the ECM

What is the gold standard for evaluating decellularization success?

• Measuring residual DNA content

• Measuring tissue weight

• Assessing tissue elasticity

• Visual inspection of color

Which of the following is a method for preserving glycosaminoglycans during decellularization?

• Adding more nucleic acids

• Increasing temperature

• Shorter exposure times

• Using harsher detergents

What is the ultimate goal of recellularization of ECM scaffolds?

• To enhance the color of the tissue

• To increase the size of the ECM

• To develop bioengineered tissue that mimics native function

• To create synthetic tissues