Operon & BioTech Review

Which of the following describes an operon that is inactive and becomes activated by a molecule binding to the repressor?

• inducible operon

• repressible operon

The lac operon is an example of an inducible operon. Which of the following describes the process that occurs when lactose is present?

• The lactose binds to the repressor which inactivates it and the repressor binds to the operator to turn translation on.

• The lactose binds to the repressor which activates it and the repressor releases from the operator to turn translation on.

• The lactose binds to the repressor which inactivates it and the repressor releases from the operator to turn translation on.

• The lactose binds to the repressor which activates it and the repressor binds to the operator to turn translation on.

Which of the following describes the function of the operator in the operon?

• site of repressor binding for on/off switch

• site of RNA polymerase binding for transcription

• site of promoter binding for RNA synthesis

• site of inducer binding for activation of pathway

Which DNA tech separates fragments by size/charge?

• Gel electrophoresis

• PCR

• Sequencing

• Transformation

What cuts the DNA prior to running gel electrophoresis?

• DNA polymerase

• Helicase

• Ligase

• Restriction enzyme

Which suspect was at crime scene?

• A

• B

• C

• D

In a gel electrophoresis…

• More numerous sizes are near the wells

• Less numerous sizes are near the wells

• Larger molecules are near the wells

• Shorter molecules are near the wells

The trp operon in E. Coli is responsible for making 5 polypeptides involved in the tryptophan synthesis pathway. When tryptophan is present, it combines with the repressor protein to block the operator. What type of operon is trp?

• Repressible

• Inducible

Tags

IST-2

The "on/off" switch for an operon is called the

• promoter
• repressor
• operator
• gene

What is the function of a plasmid?

• The site of respiration in prokaryotes
• The site of photosynthesis in eukaryotes
• The site of protein synthesis in prokaryotes and eukaryotes
• The site of hereditary material in prokaryotes