Genomics - L7

What does RNA polymerase consist of?

• A single polypeptide chain
• DNA-binding motifs
• Multiple subunits that form a core enzyme
• A ribosome and tRNA

What is a promoter?

• The enzyme that transcribes DNA
• A short RNA primer
• A DNA sequence that signals where RNA polymerase should bind
• A protein-binding domain

How does RNA polymerase use the promoter?

• To terminate transcription
• To replicate the DNA strand
• As a binding site to initiate RNA synthesis
• As a scaffold for splicing

What is transcription?

• Replication of RNA from DNA
• Synthesis of DNA from protein
• Synthesis of RNA using DNA as a template
• Translation of DNA into protein

What does initiation in transcription refer to?

• Cleavage of DNA strands
• The process of extending the RNA chain
• The process where RNA polymerase binds to promoter and begins RNA synthesis
• Translation of RNA into protein

What is elongation in transcription?

• RNA polymerase leaves the promoter and synthesizes RNA
• DNA is replicated
• Proteins are translated
• Splicing of exons occurs

What is termination in transcription?

• Binding of ribosomes to mRNA
• Cleaving exons
• Ending RNA synthesis and releasing RNA polymerase
• Removal of introns

Which of the following is NOT a subunit of E. coli RNA polymerase?

• Alpha
• Beta
• Sigma
• Theta

Which are the largest subunits of RNA polymerase?

• Alpha and Omega
• Beta and Beta'
• Sigma and Rho
• Delta and Lambda

Which RNA polymerase subunit can be separated from the rest?

• Alpha
• Beta
• Sigma
• Beta'