How to use Microscopes

If your ocular is 10x and your objective is 40x, what is the total magnification of your image?

• 400x

• 40x

• 4x

• 4000x

Why do you need a coverslip?

• The cover glass protects the microscope's eyepiece from contacting the specimen. It also creates an uneven thickness (in wet mounts) for viewing.

• The cover glass protects the microscope's objective lens from contacting hte specimen. It also creates an even thickness (in wet mounts) for viewing.

• The cover glass protects the microscope's diaphragm from contacting the specimen. It also creates an even thickness (in dry mounts) for viewing.

• The cover glass protects the microscope's base from contacting hte specimen. It also creates an uneven thickness (in dry mounts) for viewing.

When using a coverslip, how do you avoid air bubbles?

• Using a pipet, add a drop of water to a dirty microscope slide. Place the edge of a coverslip on the slide so that it touches the water. Abruptly drop the coverslip on to the slide to prevent the formation of air bubbles.

• Using a pipet, add a drop of water to a clean microscope slide. Then, place the specimen in the water. Place the edge of a coverslip on the slide so that it touches the water. Quickly lower the coverslip to prevent the formation of air bubbles.

• Place the specimen directly on the sldie. Then, place the edge of a coverslip on the slide so that it touches the specimen. Slowly lower the coverslip to prevent the formation of air bubbles.

• Using a pipet, add a drop of water to a clean microscope slide. Then, place the specimen in the water. Place the edge of a coverslip on the slide so that it touches the water. Slowly lower the coverslip to prevent the formation of air bubbles.

When storing the microscope, what objective should be locked in place?

Which focus knob provides a sharper image?

The ocular ...

• supports the slide

• roughly brings specimen to focus

• is the eye piece

• rotates objectives

The body tube

• connects the ocular to the objective

• holds slide in place

• supports the microscope

• rotates objects

The stage clip

• is the eye piece

• makes light to see the specimen

• holds the slide in place

• controls the amount of light through the aperture

The diaphragm

• controls the amount of light through the aperture

• adjusts image for fine detail

• roughly brings the specimen into focus

• supports the slide

The course adjustment knob

• connects the ocular to objective

• roughly brings specimen into focus

• needs to be held when transporting the microcscope

• adjusts image for fine detail