L3_CRISPR Technology Quiz

What is the function of the HNH nuclease domain in Cas9?

• Nick the target DNA strand

• Nick the non-target DNA strand

• Activate transcription

• Bind to sgRNA

What does the term 'Dead Cas9' (dCas9) refer to?

• A Cas9 variant that enhances gene expression

• A Cas9 variant that is catalytically inactive

• A Cas9 variant that only binds to DNA

• A Cas9 variant that can cut DNA

Which of the following is a method used for gene knockout?

• CRISPRi

• Base Editing

• CRISPRa

• CRISPR/Cas9

What does the term 'sgRNA' stand for?

• Standard guide RNA

• Short guide RNA

• Synthetic guide RNA

• Single guide RNA

Which enzyme is used in the Cytidine Base Editor (CBE)?

• Histone acetyltransferase

• DNA methyltransferase

• Cytidine Deaminase

• Adenosine Deaminase

What is the primary role of dCas9 in CRISPRi?

• To induce double strand breaks

• To silence target gene expression

• To edit DNA bases

• To activate gene expression

What is the purpose of using two independent sgRNAs in Cas9n double nicking?

• To silence gene expression

• To visualize chromatin

• To create a double strand break

• To enhance gene expression

Which of the following is a characteristic of the Cas9 Nickase (Cas9n)?

• Cuts only one DNA strand

• Binds to RNA only

• Cuts both DNA strands

• Is catalytically inactive

What is the function of the RuvC domain in Cas9?

• Nick the target DNA strand

• Nick the non-target DNA strand

• Bind to sgRNA

• Activate transcription

What is the main application of CRISPR technology in agriculture?

• Gene editing for disease resistance

• Gene editing for increased yield

• Gene editing for pest resistance

• All of the above