VEGA

Which one is correct for PacBio long-read sequencing?

• PacBio long-read sequencing uses SMRT sequencing technology

• HiFi reads are produced using the circular consensus sequencing (CCS) mode

• No PCR amplification step for library preparation

• All above

Which one is correct for HiFi sequencing advantages?

• Long read lengths up to 25 kb

• High accuracy with 99.9%

• Simultaneous epigenetic detection

• All above

 Which of the following methods used for evaluation of DNA quality?

• Qubit for DNA quantification, Nanodrop for DNA purity

• Qubit for DNA quantification and Femto Pulse system for DNA sizing

• Qubit for DNA quantification, Nanodrop for DNA purity, and Femto Pulse system for DNA sizing

• Nanodrop for both DNA quantification and DNA purity, and Femto Pulse system for DNA sizing

Which one is correct for SMRTbell library preparation?

• DNA repair and A-tailing -> Adapter ligation -> Nuclease treatment

• DNA shearing -> DNA repair and A-tailing -> Adapter ligation -> Nuclease treatment

• DNA shearing -> Bead cleanup -> DNA repair and A-tailing -> Adapter ligation -> Bead cleanup -> Nuclease treatment -> Bead cleanup

• DNA shearing -> Bead cleanup -> DNA repair and A-tailing -> Adapter ligation -> Bead cleanup -> Nuclease treatment

   

What is the Probability of Incorrect Base Call of Q40?

• 1 in 10

• 1 in 100

• 1 in 1,000

• 1 in 10,000