chromatography

Gas Chromatography Analogy: Airport Security Scanner

• Size

• Color

• Shape

• Texture

4. To analyze complex samples

• It’s used in food testing, drug testing, environmental testing, and more, to detect even tiny amounts of a substance

• It is primarily used for simple mixtures and solutions.

• It is only applicable in laboratory settings with advanced equipment.

• It is mainly used for qualitative analysis rather than quantitative.

Components of Chromatography

• Stationary Phase

• Mobile Phase

• Sample/Mixture

• Solvent

Problem no. 1: A compound was detected 6.2 minutes after injection. What is its retention time?

• 4.5 minutes

• 6.2 minutes

• 7.0 minutes

• 5.8 minutes

What is Gas Chromatography?

• A technique used to separate gases or vaporized liquids in a mixture, so we can identify or measure them.

• A method for measuring the temperature of gases in a closed system.

• A process for converting liquid samples into gas for analysis.

• A technique for mixing different gases to create a new compound.

Problem no. 4: The compound was detected at 8.5 minutes, and tₘ is 2.0 minutes. Find the: a) Adjusted retention time b) Capacity factor

• Adjusted retention time = 6.5 minutes, Capacity factor = 4.25

• Adjusted retention time = 8.5 minutes, Capacity factor = 3.25

• Adjusted retention time = 10.5 minutes, Capacity factor = 5.25

• Adjusted retention time = 7.5 minutes, Capacity factor = 3.75

4. Detector (in advanced setups)

• A tool used to spot or measure the separated substances, especially in machines like HPLC or gas chromatography

• A device that generates heat for chemical reactions

• A component that stores samples for analysis

• A mechanism that filters out impurities from substances

Mobile Phase of HPLC High Performance liquid chromatography?

• Gas

• Solid

• Liquid

• Supercritical Fluid

Stationary

Phase of TLC?

• Silica on glass

• Alumina on plastic

• Cellulose on paper

• Carbon on metal

Board work 1:

A solute’s retention time is 5.20 minutes. The mobile phase

takes 1.10 minutes to travel the column.

Find the adjusted retention time (t′ᵣ).

• 4.10 minutes

• 5.20 minutes

• 6.30 minutes

• 3.10 minutes