Nucleic Acids Amplification & Sequencing

Nucleic Acids Amplification & Sequencing

University

31 Qs

quiz-placeholder

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Nucleic Acids Amplification & Sequencing

Nucleic Acids Amplification & Sequencing

Assessment

Quiz

Chemistry

University

Practice Problem

Hard

Created by

yusoph manalundong

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31 questions

Show all answers

1.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What are the most commonly analysed nucleic acids?

Chromosomal DNA

Plasmid DNA

Messenger RNA (mRNA)

All of the above

2.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What is the first step in the isolation process of nucleic acids?

Isolation

Cell lysis

Concentration

Amplification

3.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What method can be used to isolate DNA using buoyant densities?

Centrifugation

CsCl density gradient centrifugation

Precipitation

Size exclusion chromatography

4.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What is the role of the detergent in total cellular DNA isolation?

To disrupt cellular walls

To precipitate DNA

To digest proteins

To isolate RNA

5.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What is a common method for RNA isolation?

Proteinase K method

CsCl density gradient centrifugation

Ethanol precipitation

Centrifugation

6.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What is the principle of the Polymerase Chain Reaction (PCR)?

Amplification of specific nucleic acid sequences

Isolation of DNA

Separation of RNA from DNA

Digestion of proteins

7.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What are the three temperature-controlled steps in PCR?

Denaturation, Annealing, Extension

Isolation, Amplification, Extraction

Lysis, Precipitation, Centrifugation

Digestion, Isolation, Amplification

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