Conjugation differs from reproduction because conjugation

Transfers DNA horizontally, to cells in the same generation.

Transfers DNA vertically, to new cells.

Which of the following statements is false regarding a bacterium that is R+?

It is resistant to certain drugs and heavy metals.

R+ can be transferred to a cell of the same species.

R+ can be transferred to a different species.

If you knew the sequence of nucleotides within a gene, which one of the following could you determine with the most accuracy?

The primary structure of the protein

The tertiary structure of the protein

The secondary structure of the protein

The quaternary structure of the protein

According to the operon model, for the synthesis of an inducible enzyme to occur, the

Substrate must bind to the repressor.

Repressor must not be synthesized.

Repressor must bind to the operator.

End-product must not be in excess.

Substrate must bind to the enzyme.

Synthesis of a repressible enzyme is stopped by

The corepressor-repressor binding to the operator.

The end-product binding to the promoter.

The corepressor binding to the operator.

The substrate binding to the repressor.

If you have inserted a gene in the Ti plasmid, the next step in genetic engineering is

Inserting the Ti plasmid into Agrobacterium.

Inserting the Ti plasmid into a plant cell.

Transformation of an animal cell.

Transformation of E. coli with Ti plasmid.

Which of the following processes is NOT involved in making cDNA?

RNA processing to remove introns

Gene silencing blocks an undesirable product by

Allosteric inhibition of an enzyme.

Which of the following is an advantage of using E. coli to make a human gene product?

It doesn't secrete most proteins.

Endotoxin may be in the product.

A colleague has used computer modeling to design an improved enzyme. To produce this enzyme, the next step is to

Determine the nucleotide sequence for the improved enzyme.

Mutate bacteria until one makes the improved enzyme.

Look for a bacterium that makes the improved enzyme.

Synthesize the gene for the improved enzyme.

Use siRNA to produce the enzyme.

Which of the following is NOT an agricultural product made by DNA techniques?

Bacillus thuringiensis insecticide

Nitrogenase (nitrogen fixation)

The use of an antibiotic-resistance gene on a plasmid used in genetic engineering makes

The recombinant cell unable to survive.

The recombinant cell dangerous.

The purpose of the Human Genome Project was to

Sequence the nucleotides in human DNA.

Identify all of the human genes.

Identify genes of all organisms.

E. coli makes insulin because

The insulin gene was inserted into it.

No reason; it doesn't make insulin.

It picked up the insulin gene from another cell.

It needs to regulate its cell-glucose level.

It's an ancient gene that now has no function.

Which of the following statements is false?

DNA replication proceeds in one direction around the bacterial chromosome.

DNA polymerase joins nucleotides in one direction only.

Multiple replication forks are possible on a bacterial chromosome.

The lagging strand of DNA is started by an RNA primer.

The leading strand of DNA is made continuously.

Which of the following statements about bacteriocins is false?

They cause food-poisoning symptoms.

They can be used to identify certain bacteria.

Nisin is a bacteriocin used as a food preservative.

The genes coding for them are on plasmids.

Which of the following pairs is mismatched?

RNA polymerase - makes a molecule of RNA from an RNA template

Transposase - insertion of DNA segments into DNA

DNA ligase - joins segments of DNA

DNA gyrase - coils and twists DNA

DNA polymerase - makes a molecule of DNA from a DNA template

The following steps are used to make DNA fingerprints. What is the third step?

Digest with a restriction enzyme.

Large numbers of bacterial cells are NOT found in crown galls because

A gene in plant cells is controlling growth.

The assumption is not true; many bacteria are in the galls.

Cell walls protect the plant from bacterial invasion.

PCR can be used to identify an unknown bacterium because

DNA polymerase will replicate DNA.

Subunit vaccines can be made by genetic modification of yeast cells. A side effect of these vaccines might be

Failure of the vaccine to provide immunity.

Restriction enzymes are

Bacterial enzymes that splice DNA.

Animal enzymes that splice RNA.

Viral enzymes that destroy host DNA.

Bacterial enzymes that destroy phage DNA.

In Table 8.1, what will be the result of conjugation between cultures 1 and 2?

1 will remain the same; 2 will become F + , leucine - , histidine -

1 will remain the same; 2 will become F + and recombination may occur

1 will become F - , leu + , his + ; 2 will become F + , leu - , his -

1 will become F - , leu - , his - ; 2 will remain the same

1 will remain the same; 2 will become F + , leu + , his +

In Figure 8.4, the antibiotic chloramphenicol binds the 50S ribosome as shown. From this information you can conclude that chloramphenicol

Prevents translation in prokaryotes.

Prevents transcription in prokaryotes.

Prevents transcription in eukaryotes.

Prevents mRNA-ribosome binding.

Prevents translation in eukaryotes.

(Use Table 8.2.) What is the sequence of amino acids encoded by the following sequence of bases in a strand of DNA? 3' ATTACGCTTTGC

Transcription would stop at the first codon

Leucine-arginine-lysine-alanine

Asparagine-arginine-lysine-alanine

Asparagine-cysteine-valine-serine

Microbiology Ch. 8/9

Authored by Kate Blankenship

Physics

University

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MULTIPLE CHOICE QUESTION

30 sec • 1 pt

The initial effect of ionizing radiation on a cell is that it causes

The formation of highly reactive ions.

Base substitutions.

DNA to break.

Bonding between adjacent thymines.

The cells to get hot.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

Which of the following is NOT a product of transcription?

tRNA

None of these

mRNA

A new strand of DNA

RNA

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

An enzyme that copies DNA to make a molecule of RNA is

RNA polymerase.

Transposase.

DNA polymerase.

Restriction enzyme.

DNA ligase.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

Transformation is the transfer of DNA from a donor to a recipient cell

As naked DNA in solution.

By cell-to-cell contact.

By sexual reproduction.

By a bacteriophage.

By crossing over.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

The mechanism by which the presence of arabinose controls the arabinose operon is

Repression.

Translation.

Induction.

Catabolite repression.

DNA polymerase.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

The damage caused by ultraviolet radiation is

Repaired during transcription.

Never repaired.

Repaired by DNA replication.

Repaired during translation.

Cut out and replaced.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

DNA is constructed of

None of these.

A single strand of nucleotides with internal hydrogen bonding.

Two strands of identical nucleotides with hydrogen bonds between them.

Two strands of nucleotides running antiparallel.

Nucleotides bonded A-C and G-T.

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Microbiology Ch. 8/9

The initial effect of ionizing radiation on a cell is that it causes

Which of the following is NOT a product of transcription?

An enzyme that copies DNA to make a molecule of RNA is

Transformation is the transfer of DNA from a donor to a recipient cell

The mechanism by which the presence of arabinose controls the arabinose operon is

The damage caused by ultraviolet radiation is

DNA is constructed of

Conjugation differs from reproduction because conjugation

Repair of damaged DNA might be viewed as a race between an endonuclease and

The cancer gene ras produces mRNA containing an extra exon that includes a number of UAA codons. Cancer cells produce ras mRNA missing this exon. This mistake most likely is due to a mistake by

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