Tracer Technique Quiz

Which detector uses low-pressure gas to detect ionizing radiation?

• NMR Spectrophotometer

• Gas Ionization Chamber

• GM Counter

• Autoradiography

The labelled precursor is introduced into plants in the precursor-product sequence method to study:

• Metabolic disorders

• Rate of photosynthesis

• Biogenesis of plant constituents

• pH change in tissues

Which technique gives information about bond cleavage and formation?

• Isotope incorporation

• Double labelling

• Competitive feeding

• Grafting

In competitive feeding, which plant alkaloids are studied using ¹⁴C compounds?

• Tropane alkaloids

• Caffeine alkaloids

• Steroidal alkaloids

• Glycoalkaloids

What is the primary use of the autoradiography technique?

• To determine isotope mass

• To study radiation emission

• To visualize radioactive material distribution

• To analyze protein folding

Tritium is produced by:

• Gamma irradiation

• Neutron bombardment of lower hydrogen isotopes

• Electron transfer reactions

• DNA synthesis

What must be considered while selecting a tracer compound?

• Must be yellow in color

• Must be non-reactive

• Must have high half-life and active participation in synthesis

• Should degrade quickly

What kind of compound is used to study glucose in biological systems?

• ¹⁵N-labelled nitrate

• ¹⁴C-labelled glucose

• ³²P-labelled ATP

• ³⁵S-labelled protein

What is the purpose of using double or multiple labelling techniques?

• To purify alkaloids

• To identify nature of precursor incorporation

• To destroy radioisotopes

• To measure chlorophyll content

What is the significance of isotope incorporation technique?

• Identification of enzyme structure

• Understanding cleavage and bond formation in reactions

• Quantifying pigment concentration

• Increasing plant biomass