Histology lecture 1 session 2 quiz

Which of the following best describes the staining properties of Hematoxylin?

• Acidic dye that stains basic structures pinkish red

• Basic dye that stains acidic structures purplish blue

• Neutral dye that stains all cellular components equally

• Basic dye that stains basic structures purplish blue

What is the primary function of Eosin in H&E staining?

• Stains nuclei blue by binding to DNA

• Stains acidic macromolecules pink

• Stains basic cytoplasmic organelles, proteins and collagen pinkish red

• Stains lipids and carbohydrates green

Which cellular structures are most prominently stained by Hematoxylin?

• Mitochondria and lysosomes

• Plasma membrane and cytoskeleton

• Golgi apparatus and smooth ER

• Nuclei and rough endoplasmic reticulum

What color does Eosin typically impart to the structures it stains?

• Yellow

• Green

• Purplish blue

• Pinkish red

Why are Hematoxylin and Eosin commonly used together in histological staining?

• They both stain the same structures for enhanced contrast

• They provide complementary staining of acidic and basic structures

• Hematoxylin enhances the effect of Eosin

• They are both acidic dyes that stain all cell components

Which of the following is NOT typically stained by Eosin?

• DNA in the nucleus

• Collagen

• Mitochondria

• Cytoplasmic proteins

What is the primary purpose of Immunohistochemistry (IHC)?

• To stain all cell structures uniformly

• To visualize DNA and RNA in cells

• To detect specific antigens in tissue sections using antibodies

• To measure enzyme activity in blood samples

What does the colored reaction in IHC indicate?

• The presence of DNA

• The location of specific antigens

• The presence of mitochondria

• The pH level of the tissue

What type of stain is Pararosaniline–Toluidine Blue classified as?

• Combination stain

• Simple stain

• Differential stain

• Negative stain

In Pararosaniline–Toluidine Blue staining, what color does chromatin appear?

• Red

• Light violet

• Purple

• Green