Eosin methylene blue agar is considered a: A) differential media B) selective media

c. If associated with both A and B

d. If not associated with both A and B

In the E-Test, how is the MIC determined?

a. Where the growth ellipse intersects the E-Test

b. By measuring the zone of inhibition

c. By comparing to a standard chart

The gold standard for MRSA detection is the detection of the:

b. Mec A gene by using PCR amplification

a. Sphingomyelinase C by using PCR amplification

c. Culture and Sensitivity testing

e. Gram stain followed by Culture and Sensitivity

What is the treatment of choice for MRSA?

d. Trimethoprim sulfamethoxazole

All of the following are characteristic of Staphylococcus saprophyticus except:

a. UTIs in young sexually active women

b. Species adheres more effectively to the epithelial cells lining the urogenital tract than other Coagulase negative Staphylococcus spp.

c. Present in urine culture with <10,000 CFU/mL and still be significant

Oxidation/Fermentation reactions produced in O/F glucose medium

d. Is to differentiate Staphylococcus to micrococcus

a. Is to differentiate Staphylococci to Streptococci

b. Is to differentiate Staphylococcus aureus to Staphylococcus epidermidis

c. Is to differentiate the virulence factors of Staphylococci

All of the following are characteristic of Staphylococcus saprophyticus except:

a. UTIs in young sexually active women

b. Species adheres more effectively to the epithelial cells lining the urogenital tract than other Coagulase negative Staphylococcus spp.

c. Present in urine culture with <10,000 CFU/mL and still be significant

Which of the following incorrectly describes Streptolysin O (SLO)?

b. Is active only in an aerobic environment

c. Able to lyse not only RBCs but also leukocytes, platelets, and other cells

d. May be detected using the ASO test

Which of the following virulence factors is correctly paired with its pathogenic capabilities?

a. Hyaluronidase - solubilizes hyaluronic acid to separate the tissues and help spread the infection

b. F protein - prevents phagocytosis

c. Streptokinase - creates fibrin clots

d. Streptolysin O - surface hemolysis

Novobiocin is used to differentiate S. epidermidis from S. saprophyticus. Which of the following result is correctly matched?

a. S. saprophyticus = resistant

c. Both organisms are susceptible

It resembles mariner's wheel and gives a "mickey mouse cap" appearance to the yeast cell.

b. Paracoccidioides brasilliensis

Which of the following clinical forms of Streptococcus pyogenes infections is being described?: This is an invasive infection characterized by rapidly progressing inflammation and localized death of living tissues of the skin, subcutaneous fat, and fascia.

e. Streptococcal Toxic Shock Syndrome

Which of the following incorrectly describes Enterococci?

a. they exhibit a true positive catalase test reaction

b. most species are non-hemolytic, some strains may show beta-hemolysis

c. frequently causes nosocomial infections, with UTI being the most common

Which of the following is incorrect for C. perfringens?

d. Double zone of hemolysis on anaerobic BAP

Which of the following is NOT true for Yersinia pestis?

a. Bipolar staining: safety pin appearance

b. Stalactite pattern on culture broth

Which of the following viruses have helical capsid symmetry?

c. Influenza virus, Parainfluenza virus, Hepatitis D virus

a. Norwalk virus, Rotavirus, Hepatitis A virus

d. Poliovirus, Hepatitis E virus, Rift Valley Fever Virus

Which of the following are naked viruses?

a. Poliovirus, Hepatitis E virus, Rotavirus

b. Parvovirus B-19, Hepatitis B Virus, HSV 1

c. HIV, Hepatitis A virus, Hepatitis B virus

d. Adenovirus, Influenza virus, mumps

What is the most likely pathogen related to rat-bite fever?

c. Treponema pallidum subsp. endemicum

Tween 80 hydrolysis is the most useful for identification of:

c. M. avium-intracellulare complex

To prevent the contamination of microscopes and surrounding areas disenfect/clean used slides, prepared by student, with

C. 5% methylene blue and lens paper

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MULTIPLE CHOICE QUESTION

30 sec • 1 pt

Which of the following does NOT match? (1) Spirochetes = Spiral (2) Bacilli = Spherical (3) Gram-positive = Blue to purple (4) Gram-negative = Pink

a. 1 only

b. 2 only

c. 3 only

d. 4 only

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

Staphylococcal leucocidin ____ is an exotoxin lethal to polymorphonuclear leukocytes. It has been implicated as contributing to the invasiveness of the organism by suppressing phagocytosis and has been associated with severe cutaneous infections and necrotizing pneumonia.

a. Alpha hemolysin

b. Beta hemolysin

c. Delta hemolysin

d. Panton - valentine leukocidin

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

All statements are true regarding JEMBEC system, except:

a. Can be used as a transport system for vaginal discharge

b. Contains selective agar and a N2

c. Can be used as a transport system for urethral discharge

d. A and C

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

Inhibitors contained in Salmonella-Shigella agar:

a. Gentian violet

b. Sodium azide

c. Bile salts and brilliant green

d. Potassium tellurite

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

Phenylethyl alcohol is used to:

a. Inhibit gram-negative bacteria

b. Inhibit gram-positive bacteria

c. Both A and B

d. None of the above

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

Which of the following is/are TRUE with Thayer-Martin agar? I. Thayer-Martin agar is a selective media used for the isolation of Neisseria gonorrhoeae II. It uses a combination of Vancomycin, Colistin, and Nystatin (VCN) ti select out N. gonorrhoeae III. Modified Thayer Martin agar uses trimethoprim in addition. IV. Thayer-Martin agar is a nonselective media used for the isolation of Neisseria gonorrhoeae

a. I only

b. I and II

c. I, II, and III

d. I, II, III, and IV

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

Which media should be used to recover Bordetella pertussis from a nasopharyngeal specimen?

a. CHocolate agar

b. Blod agar

c. Bordet-Gengou agar

d. MacConkey Agar

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MTQap_Qrationale

Which of the following does NOT match? (1) Spirochetes = Spiral (2) Bacilli = Spherical (3) Gram-positive = Blue to purple (4) Gram-negative = Pink

Staphylococcal leucocidin ____ is an exotoxin lethal to polymorphonuclear leukocytes. It has been implicated as contributing to the invasiveness of the organism by suppressing phagocytosis and has been associated with severe cutaneous infections and necrotizing pneumonia.

All statements are true regarding JEMBEC system, except:

Inhibitors contained in Salmonella-Shigella agar:

Phenylethyl alcohol is used to:

Which media should be used to recover Bordetella pertussis from a nasopharyngeal specimen?

Hemolytic Streptococci require blood to support their growth. What type of media should be used to cultivate such bacteria?

These media allow only the growth of desired organisms while inhibiting the others.

These media can be used to distinguish lactose-fermenting and non-lactose-fermenting bacteria in terms of color reaction.

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