Chemical method; its principle is colorimetry

Uricase (coupled enzyme method)

Isotope dilution mass-spectrometry

Most common method BUT lacks specificity

Uricase (coupled enzyme method)

Isotope dilution mass-spectrometry

Protein-free filtrate -> uric acid + phosphotungstic acid -alkaline solution -> tungsten blue

Uricase (coupled enzyme method)

Isotope dilution mass-spectrometry

chemical method; NOT SPECIFIC!!!!!!

Uricase (coupled enzyme method)

Isotope dilution mass-spectrometry

simplest and most specific method <3<3<3

Uricase (coupled enzyme method)

Isotope dilution mass-spectrometry

Principle: uric acid + uricase --> oxidation (ALLANTOIN) + H2O2

Uricase (coupled enzyme method)

Isotope dilution mass-spectrometry

Reference range: Males = 3.5 to 7.2 mg/dL Females - 2.6 to 6 m/dL

Uricase (coupled enzyme method)

Isotope dilution mass-spectrometry

Decrease in absorbance is monitored at 293 nm

Uricase (coupled enzyme method)

Isotope dilution mass-spectrometry

Uric acid = peak absorbance at 293 nm Allantoin = no absorbance at 293 nm

Uricase (coupled enzyme method)

Isotope dilution mass-spectrometry

Uric acid -uricase -> allantoin & H2O2

Uricase (coupled enzyme method)

Isotope dilution mass-spectrometry

H2O2 + chromogen -peroxidase -> oxidized chrmogen

Uricase (coupled enzyme method)

Isotope dilution mass-spectrometry

Uricase (coupled enzyme method)

This model proposed that enzymes recognize their substrates as a lock receives a key. Basically, this theory states that enzymes have a specific shape that directly correlates to the shape of the substrate.

Emil Fischer / Lock and Key theory

According to this theory, binding of substrate to the enzyme brings a conformational change in the enzyme to either enhance or inhibit its activity.

Emil Fischer / Lock and Key theory

PRCC_4MT-P1

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1.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

Purine catabolism

Creatine

Blood Uric Acid

Blood Urea Nitrogen

Bilirubin

2.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

>95% exists as monosodium urates

Creatine

Blood Uric Acid

Blood Urea Nitrogen

Bilirubin

3.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

98 to 99% are reabsorbed in the proximal convoluted tubules (PCT)

Creatine

Blood Uric Acid

Blood Urea Nitrogen

Bilirubin

4.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

Blood Uric acid becomes _ in liver damage

High

Low

5.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

leukemia

Increased nuclear metabolism and cell turn over

Other causes of hyperuricemia

Decreased uric acid (hypouricemia)

6.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

hemolytic disease

Increased nuclear metabolism and cell turn over

Other causes of hyperuricemia

Decreased uric acid (hypouricemia)

7.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

lymphoma

Increased nuclear metabolism and cell turn over

Other causes of hyperuricemia

Decreased uric acid (hypouricemia)

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PRCC_4MT-P1

157 questions

Purine catabolism

>95% exists as monosodium urates

98 to 99% are reabsorbed in the proximal convoluted tubules (PCT)

Blood Uric acid becomes _ in liver damage

leukemia

hemolytic disease

lymphoma

multiple myeloma

polycythemia

megaloblastic anemia

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