
Immunotech and Molecular Biology Quiz
Authored by Asst Prof Dr. Shivani Khatri BT
Biology
University
Used 4+ times

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12 questions
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1.
OPEN ENDED QUESTION
30 sec • Ungraded
Name:
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2.
OPEN ENDED QUESTION
30 sec • Ungraded
Roll no.
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3.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
Antibody-antigen interactions rely mainly on non-covalent forces. If you wanted to experimentally disrupt hydrogen bonds specifically during an antigen-antibody binding assay, which treatment would most likely affect the binding affinity?
Increasing ionic strength with NaCl
Altering the pH of the buffer
Adding a mild detergent like Triton X-100
Reducing the temperature
4.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
You are designing a diagnostic test to detect an early infection when the antigen is present in very low amounts. Which assay would provide the highest sensitivity?
Direct ELISA
Radioimmunoassay (RIA)
Western blot
Sandwich ELISA
5.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
In a Western blot, an unexpected band is observed in a patient sample at a lower molecular weight than the target protein. Which of the following most plausibly explains this observation?
Cross-reactivity of the antibody with a degradation product
Incomplete transfer of the protein onto the membrane
Over-saturation of the substrate in the detection step
Failure of blocking step leading to non-specific binding
6.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
You are comparing two techniques for cytokine quantification: ELISPOT and ELISA. Which of the following statements is correct?
ELISPOT provides information about total cytokine concentration, while ELISA shows cytokine-secreting cell frequency
ELISA is more sensitive than ELISPOT in detecting rare cytokine-producing cells
ELISPOT can quantify cytokine production at the single-cell level, while ELISA measures cumulative secretion in a bulk population
Both techniques provide identical information but use different detection labels
7.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
Which of the following best explains why maternal antibodies transferred to a newborn provide only temporary protection?
They are rapidly degraded by lysosomal enzymes
They do not stimulate memory cell formation in the infant
They compete with infant antibodies for Fc receptors
They enhance tolerance rather than protection
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