Rate Limiting Enzme for Glycolysis

• Phosphofructokinase

• Fructose 1,6-bisphosphatase

• Glycogen synthase

• Glycogen phosphorylase

Rate Limiting Enzme for Gluconeogenesis

• Phosphofructokinase

• Fructose 1,6-bisphosphatase

• Glycogen synthase

• Glycogen phosphorylase

Rate Limiting Enzme for Glycogenesis

• Phosphofructokinase

• Fructose 1,6-bisphosphatase

• Glycogen synthase

• Glycogen phosphorylase

Rate Limiting Enzme for Glycogenolysis

• Phosphofructokinase

• Fructose 1,6-bisphosphatase

• Glycogen synthase

• Glycogen phosphorylase

Rate Limiting Enzyme for Citric Acid Cycle

• Isocitrate dehydrogenase

• Glucose-6-phosphate dehydrogenase

• Acetyl CoA carboxylase

• Carnitine-palmitoyl transferase

• Carbamoyl phosphate synthetase 1

Rate Limiting Enzyme for Pentose Phosphate Pathway / HMP Shunt

• Isocitrate dehydrogenase

• Glucose-6-phosphate dehydrogenase

• Acetyl CoA carboxylase

• Carnitine-palmitoyl transferase

• Carbamoyl phosphate synthetase 1

Rate Limiting Enzyme for Lipogenesis

• Isocitrate dehydrogenase

• Glucose-6-phosphate dehydrogenase

• Acetyl CoA carboxylase

• Carnitine-palmitoyl transferase

• Carbamoyl phosphate synthetase 1

Rate Limiting Enzyme for Lipolysis / Beta Oxidation

• Isocitrate dehydrogenase

• Glucose-6-phosphate dehydrogenase

• Acetyl CoA carboxylase

• Carnitine-palmitoyl transferase

• Carbamoyl phosphate synthetase 1

Rate Limiting Enzyme for Urea Cycle

• Isocitrate dehydrogenase

• Glucose-6-phosphate dehydrogenase

• Acetyl CoA carboxylase

• Carnitine-palmitoyl transferase

• Carbamoyl phosphate synthetase 1

Tests for Free Amino Acid

• Ninhydrin

• Xanthoproteic

• Millon’s

• Hopkin’s Cole

• Sakaguchi