W3-4 Gene cloning Recap

When human DNA is inserted in bacterial DNA to create insulin it is called

• viral DNA
• bacterial DNA
• recombinant DNA
• transcribed DNA

Tags

NGSS.HS-LS1-1

What is necessary for traditional gene cloning?

• Restriction enzymes

• DNA ligase

• DNA primers

• DNA Polymerase

What method(s) did we use to transform bacteria with recombinant plasmid?

• Heat Shock

• Electroporation

• Freeze shock

• Calcium Chloride treatment

What is the purpose of streaking transformed bacteria on antibiotic agar plate? (choose all correct answers)

• To amplify the recombinant DNA

• To screen for successful clones containing the recombinant plasmid

• To isolate single colony for further growth

• To improve the efficiency of transformation

What are the possible applications of gene cloning?

• Adding mutations to the gene of interest

• Production of fusion proteins

• Production of recombinant protein

• Gene knockout

Tags

NGSS.HS-LS1-1

In gel electrophoresis, the largest DNA fragment will appear

• closest to the starting wells
• farthest from the starting wells
• three quarters away from the starting wells
• it depends on how many fragments there are

Tags

NGSS.MS-PS2-5

Which statements about gel electrophoresis are true?

• DNA ladder represents sizes of linear DNA

• Blue gel loading dye is necessary for being able to see the sample during gel electrophoresis

• SYBR Safe or Ethidium Bromide make DNA detectable under UV light

• Nicked relaxed plasmid appears lower on the DNA gel than the linear DNA of the same size