Laboratory Test for Secondary Hemostasis

Involves in the series or cascade on how, where and when the CF activates.

3 Distinctive Stages of Coagulation

• Stage 1: Generation of Plasma Thromboplastin

• Stage 2: Generation of Thrombin

• Stage 3: Conversion of Fibrinogen to Fibrin

• Intrinsic

• Extrinsic

Test for Stage 1: GENERATION OF PLASMA THROMBOPLASTIN

• PCT (Prothrombin Consumption Test)

• APTT (Activated Partial Thromboplastin Time)

• Mixing Studies

• Quick’s One - stage Prothrombin Time/ Protime (PT)

• Prothrombin and Proncinvertin Time Test (P and P Test)

Test for Stage 1: GENERATION OF PLASMA THROMBOPLASTIN

• Thrombotest

• Fibrometer

• Stypven Time

• TGT (Thromboplastin Generation Time)

• Hicks - Pitney Kaolin Modification of TGT

Test for Stage 1: GENERATION OF PLASMA THROMBOPLASTIN

• Known as Serum Prothrombin Time

• PCT (Prothrombin Consumption Test)

• TGT (Thromboplastin Generation Time)

• Hicks - Pitney Kaolin Modification of TGT

Test for Stage 1: GENERATION OF PLASMA THROMBOPLASTIN

• Measures the time it takes for fibrin strands to form when optimal amounts of F1, thromboplastin, calcium and serum are fixed.

• PCT (Prothrombin Consumption Test)

• TGT (Thromboplastin Generation Time)

• Hicks - Pitney Kaolin Modification of TGT

Test for Stage 1: GENERATION OF PLASMA THROMBOPLASTIN

• Measures the amount of rate of formation of prothrombinase through the intrinsic coagulation pathway.

• PCT (Prothrombin Consumption Test)

• TGT (Thromboplastin Generation Time)

• Hicks - Pitney Kaolin Modification of TGT

Test for Stage 1: GENERATION OF PLASMA THROMBOPLASTIN

• Simplified modification of the TGT which allows routine screening tests.

• PCT (Prothrombin Consumption Test)

• TGT (Thromboplastin Generation Time)

• Hicks - Pitney Kaolin Modification of TGT

Test for Stage 2: GENERATION OF THROMBIN

• Quick’s One - stage Prothrombin Time/ Protime (PT)

• Prothrombin and Proncinvertin Time Test (P and P Test)

• Thrombotest

• Whole Blood Clot Lysis

• Fibrindex

Test for Stage 2: GENERATION OF THROMBIN

• Thrombin Time

• Fibrinogen Titer

• Two - Stage Prothrombin Time

• Stypven Time

• Fibrometer